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Lignocellulosic materials, like eucalyptus wastes, are abundant and renewable cellulose and hemicellulose sources that can be used for several chemical compounds production lyke liquid fuels and chemical substances of industrial interest, including 2,3 butanediol. This alcohol can be used as a non fossil liquid fuel, which represents environmental advantages. One of the main problems related to the use of hemicellulosic hydrolysates is the presence of several toxic compounds, which are inhibitors of the microbial metabolism. This problem makes necessary the application of a treatment method for removing inhibitors or reducing their level. An alternative is to adapt the microrganism to the inhibitors, which can be achieved by conducting the fermentation in fed batch system. In this study we used the repeated fed batch system to increase the process productivity, by reusing cells already adapted to the environment and in production phase. Initially we determined the time of fermentation for the first cycle, which corresponded to the time when the substrate concentration reached 20 g/L, after the end of feeding. At this point a volume equal to the volume of medium to be added during feeding (750 mL) was removed. Then a new cycle of feeding began. Four cycles of unloading and feeding were performed. The study was conducted employing medium prepared with hemicellulosic hydrolyzate treated with activated charcoal and treated with ion exchange resin. The results showed that the cell reuse strategy was not adequate for the hydrolyzate treated with activated charcoal, whereas there was a progressive accumulation of substrate in each fermentation cycle. For the medium prepared with hydrolyzate treated with ion exchange resin there was an improvement of results with successive cycles of feeding and unloading (the product concentration at the unloading time increased from 15.76 g/L in the first cycle to 34.97 g/L in the fourth cycle).